Recombinant Human NECTIN2 Protein(DDDDK tag) (HH0080NL)

Recombinant Human NECTIN2 Protein (Q92692) (Met 1-Leu 360) with quinary-aa peptide (DDDDK) at the C-terminus was expressed in HEK293.

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PRODUCT INFORMATION

Cat.No.

HH0080NL

Synonyms

NECTIN2; Nectin-2; CD112; HVEB; PRR2; PVRL2;

Accession

NECTIN2

Predicted N Terminal

Gln 32

Form

Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized human CD112 at 10 μg/ml (100 μl/well) can bind human DNAM1-Fch with a linear range of 1.25-10 μg/ml.

Molecular Mass

Recombinant Human CD112 comprises 335 amino acids and has a calculated molecular mass of 36.2 kDa. As a result of glycosylation, Recombinant protein migRates as an approximately 48 kDa protein in SDS-PAGE under reducing conditions.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Background

LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.

Tag

DDDDK

Species

Human

Source

HEK293

BACKGROUND

Background