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Recombinant Human LIF Protein (HE0006LL)

Recombinant Human LIF Protein (P15018) (180 amino acids) with no tag was expressed in E.coli.

Size Price Qty
25ug $319.00
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PRODUCT INFORMATION

Cat.No.
HE0006LL
Synonyms
LIF; CDF; DIA; HILDA; MLPLI;
Accession
LIF
Form
Lyophilized from sterile PBS, pH 7.4.
Bio-activity
The ED50 as determined by the dose-dependent proliferation of human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of> 1.0 × 10^7 IU/mg.
Molecular Mass
Recombinant Human LIF Protein is a single non-glycosylated polypeptide chain and has a molecular mass of approximately 19.7 kDa.
AA Sequence
SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 98 % by SDS-PAGE.
Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Dissolve with double distilled water.
Warning
For research use only!
Background
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
Species
Human
Source
E. coli

BACKGROUND

Background
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.
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