Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its binding ability in a functional ELISA. Immobilized human Leptin at 5 μg/ml (100 μl/well) can bind human Leptin receptor with a linear range of 0.032-4.0 μg/ml.
Molecular Mass
Recombinant Human LEPR consists of 829 amino acids and has a predicted molecular mass of 95 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh LEPR is approximately 130-140 kDa due to glycosylation.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 95 % by SDS-PAGE.
Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.
Warning
For research use only!
Background
The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. Required for normal immune responses (PubMed:15546002, PubMed:8490660).
Tag
His
Species
Human
Source
HEK293
BACKGROUND
Background
The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. Required for normal immune responses (PubMed:15546002, PubMed:8490660).
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