Recombinant Human LCN2 Protein(His tag) (HH0104LL)

Cat.No.

HH0104LL

Synonyms

LCN2; lipocalin 2; lipocalin 2 (oncogene 24p3); NGAL; p25; MSFI;

Accession

LCN2

Predicted N Terminal

Gln 21

Form

Lyophilized from sterile PBS, pH 7.5, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by its ability to bind Iron(III) dihydroxybenzoic acid [Fe(DHBA)3]. The binding of Fe(DHBA)3 results in the quenching of Trp fluorescence in recombinant human Lipocalin-2. Recombinant human Lipocalin-2 can bind>1.5 μM of Fe(DHBA)3 .

Molecular Mass

Recombinant Human LCN2 consists of 195 amino acids. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhLCN2 is approximately 22 kDa as predicted.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 97 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Background

Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5.

Tag

His

Species

Human

Source

HEK293

Background

Functions in the lectin pathway of complement, which performs a key role in innate immunity by recognizing pathogens through patterns of sugar moieties and neutralizing them. The lectin pathway is triggered upon binding of mannan-binding lectin (MBL) and ficolins to sugar moieties which leads to activation of the associated proteases MASP1 and MASP2. Functions as an endopeptidase and may activate MASP2 or C2 or directly activate C3 the key component of complement reaction. Isoform 2 may have an inhibitory effect on the activation of the lectin pathway of complement or may cleave IGFBP5.