Human Kallikrein-1 active Unit Definitin: 1 unit will hydrolyze 1 µmole of H-D-Val-Leu-Arg-pNA(S-2266) per minute at pH 8.0 at 37 centigrade Human Kallikrein-1 measured biological activity of no less than 5 Units/mg (S2266 method).
Molecular Mass
28-32 kDa
Protein length
238 amino acids
AA Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ile-Val-Gly-Gly-Trp-Glu.
Purity
Greater than 98.0% as determined by both: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Storage
Lyophilized KLK1 although stable at room temperature for 3 weeks, should be stored desiccated below -18 centigrade. Upon reconstitution KLK1 should be stored at 4 centigrade between 2-7 days and for future use below -18 centigrade. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Reconstitution
It is recommended to reconstitute the lyophilized KLK1 in sterile 18M-cm H2O not less than 100 µg/ml or more than 10 mg/ml solutions.
Warning
For research use only!
Species
Human
Source
Pichia Pastoris
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