Recombinant Human IL7R Protein(Fc-His tag) (HH0157IL)

Recombinant Human IL7R Protein (P16871) (Met 1-Gly 236) with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus was expressed in HEK293.

Datasheet

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PRODUCT INFORMATION

Cat.No.

HH0157IL

Synonyms

IL7R; CD127; IL-7RA; ILRA; IL7RA; CDW127; IL-7R-alpha;

Accession

IL7R

Predicted N Terminal

Glu 21

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by its ability to bind biotinylated mouse TSLP-his in functional ELISA. Measured by its binding ability in a functional ELISA. Immobilized human IL7 at 10 μg/ml (100 μl/well) can bind human IL7Ra-Fch, The EC50 of human IL7Ra-Fch is 15.2-35.6 ng/m

Molecular Mass

Recombinant Human IL7Rα/Fc is a disulfide-linked homodimer. The reduced monomer consists of 464 amino acids and has a predicted molecular mass of 53 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh IL7Rα/Fc monomer is approximately 65-75 kDa due to glycosylation.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Tag

Fc-His

Species

Human

Source

HEK293