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Recombinant Human HMGB1 Protein(His tag) (HH0111HL)

Recombinant Human HMGB1 Protein (P09429) (Met 1-Glu 215) with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus was expressed in HEK293.

Datasheet

Size Price Qty
50ug $396.00
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PRODUCT INFORMATION

Cat.No.
HH0111HL
Synonyms
HMGB1; HMG3; SBP 1; HMG-1; HMG1; SBP-1;
Accession
HMGB1
Predicted N Terminal
Met 1
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.
Bio-activity
Measured by its binding ability in a functional ELISA. Immobilized human HMGB at 2 μg/ml (100 μl/well) can bind human AGER. The EC50 of human AGER is 0.27 μg/ml.
Molecular Mass
Recombinant Human HMGB1 consists of 226 amino acids and has a predicted molecular mass of 26.3 kDa. As a result of glycosylation, the apparent molecular mass of rhHMGB1 is approximately 30-34 kDa in SDS-PAGE under reducing conditions.
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method).
Purity
> 93 % by SDS-PAGE.
Storage
In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.
Reconstitution
Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.
Warning
For research use only!
Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Tag
His
Species
Human
Source
HEK293

BACKGROUND

Background
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
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