Recombinant Human ERN1 Protein(Gly & Pro tag) (HI0197EL)

Recombinant Human ERN1 Protein (O75460) (Pro 465-Leu 977) with two additional amino acids (Gly & Pro) at the N-terminus was expressed in Insect cells.

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PRODUCT INFORMATION

Cat.No.

HI0197EL

Synonyms

ERN1; IRE1; IRE1P; ire1-alpha; IRE1a; hIRE1p

Accession

ERN1

Predicted N Terminal

Gly

Form

Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4.

Bio-activity

Kinase activity untested. Measured by its nuclease activity to cleave Xbp1 single stem-loop mini-substrate.

Molecular Mass

Recombinant Human ERN1 consists of 515 amino acids and predicts a molecular mass of 58.3 KDa. The apparent molecular mass of the protein is approximately 65 KDa in SDS-PAGE under reducing conditions due to glycosylation.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 80 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Background

Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Tag

Gly & Pro

Species

Human

Source

Insect cells

BACKGROUND

Background