Recombinant Human EPCAM Protein(His tag) (HH0137EL)

Recombinant Human EPCAM Protein (P16422) (Met 1-Lys265) with a polyhistidine tag at the C-terminus was expressed in HEK293.

Datasheet

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PRODUCT INFORMATION

Cat.No.

HH0137EL

Synonyms

EPCAM; CD326; CO 17A; EGP 2; EGP34; EGP40; Ep CAM; ESA; TACST 1; TROP1; M4S1; MK-1; DIAR5; EGP-2; MIC18; EGP314; HNPCC8; TACSTD1; GA733-2;

Accession

EPCAM

Predicted N Terminal

Gln 24

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When cells are added to EpCAM-His coated plates (1.25μg/mL, 100μL/well), approximately>40% will adhere specifically after 30 minutes a

Molecular Mass

Recombinant Human EpCAM consists of 253 amino acids after removal of the signal peptide and has a calculated molecular mass of 29 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh EpCAM is approximately 36 kDa due to glycosylation.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 96 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Background

Induces cartilage and bone formation (PubMed:3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed:24362451).

Tag

His

Species

Human

Source

HEK293

BACKGROUND

Background