Recombinant Human EGF Protein (HE0009EL)

Cat.No.

HE0009EL

Synonyms

EGF; beta-urogastrone; URG; HOMG4;

Accession

EGF

Form

Lyophilized from sterile PBS, pH 7.4.

Bio-activity

The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 0.1 ng/ml, corresponding to a specific activity of> 1.0 × 10^7 IU/mg.

Molecular Mass

Recombinant Human EGF Protein is a single non-glycosylated polypeptide chain and has a molecular mass of approximately 6.2 kDa.

AA Sequence

NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 98 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Dissolve with double distilled water.

Warning

For research use only!

Background

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).

Species

Human

Source

E. coli

Background

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941).