Form
Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% Trehalose.
Bio-activity
Immobilized Human CTLA-4, Fc Tag at 10 μg/mL (100 ul/well), can bind Human B7-1, His Tag with a linear range of 0.04-4 μg/mL.
Immobilized Human B7-2, His Tag at 10 μg/mL (100 ul/well), can bind Human CTLA-4, Fc Tag with a linear range of 0.04-0.3 μg/mL.
Molecular Mass
Recombinant Human CTLA4 Protein carries a human IgG1 Fc tag at the C-terminus, and has a calculated MW of 40 kDa. As a result of glycosylation, the reducing protein migrates as 50-55 kDa, and the non-reducing protein migrates as 100-110 kDa in SDS-PAGE.
Background
Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.