Recombinant Human CD80 Protein(His tag) (HH0140CL)

Recombinant Human CD80 Protein (P33681) (Met 1-Asn 242) with a polyhistidine tag at the C-terminus was expressed in HEK293.

Datasheet

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PRODUCT INFORMATION

Cat.No.

HH0140CL

Synonyms

CD80; B7; BB1; B7-1; LAB7; CD28LG; CD28LG1;

Accession

CD80

Predicted N Terminal

Val 35

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by its ability to bind recombinant human CD28 in a functional ELISA.

Molecular Mass

Recombinant Human B7-1 consists of 219 amino acids and has a predicted molecular mass of 25.4 kDa. By reduced SDS-PAGE, the apparent molecular mass of rh B7-1 is approximately 45-48 kDa due to glycosylation.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Background

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.

Tag

His

Species

Human

Source

HEK293

BACKGROUND

Background