Recombinant Human CD74 Protein(His tag) (HH0173CL)

Recombinant Human CD74 Protein (P04233) (Gln 73-Met 232) with a polyhistidine tag at the N-terminus was expressed in HEK293.

Datasheet

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PRODUCT INFORMATION

Cat.No.

HH0173CL

Synonyms

CD74; p33; HLA-DR-gamma; DHLAG; HLADG; Ia-GAMMA

Accession

CD74

Predicted N Terminal

Val 19

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized human CD74 at 5 μg/ml (100 μl/well) can bind biotinylated human CTSL1 with a linear range of 3.2-400 ng/ml.

Molecular Mass

Recombinant Human CD74 consists of 176 amino acids and has a calculated molecular mass of 20.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCD74 is approximately 34 kDa due to glycosylation.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 80 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Background

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.

Tag

His

Species

Human

Source

HEK293

BACKGROUND

Background