Recombinant Human CD3E&CD3D Protein(His & Flag tag) (HH0036CL)

Recombinant Human CD3E Protein (P07766) (Asp 23 - Asp 126) with his-tag at the C-terminus and Human CD3D Protein (P04234) (Phe 22 - Ala 105) with flag-tag at the C-terminus was co-expressed in HEK293.

Datasheet

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PRODUCT INFORMATION

Cat.No.

HH0036CL

Synonyms

CD3E; CD3-epsilon; T3E; TCRE; CD3D;

Accession

CD3E & CD3D

Predicted N Terminal

Asp 23 (CD3E) & Phe 22 (CD3D)

Form

Lyophilized from sterile PBS, pH7.4, 10% Trehalose.

Molecular Mass

Recombinant Human CD3E&CD3D heterodimer protein is produced by co-expression of CD3E and CD3D, has a calculated MW of 16.4 kDa (CD3E) and 14.4 kDa (CD3D). The reducing heterodimer protein migrates as 20 kDa and 22-28 kDa due to glycosylation.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Dissolve with double distilled water.

Warning

For research use only!

Background

The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. Required for normal immune responses (PubMed:15546002, PubMed:8490660).

Tag

His & Flag

Species

Human

Source

HEK293

BACKGROUND

Background

The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. Required for normal immune responses (PubMed:15546002, PubMed:8490660).