Recombinant Human CD28 Protein(Fc tag) (HH0186CL)

Recombinant Human CD28 Protein (P10747) (Met 1-Pro 152) with the Fc region of human IgG1 at the C-terminus was expressed in HEK293.

Datasheet

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PRODUCT INFORMATION

Cat.No.

HH0186CL

Synonyms

CD28; CD28 antigen (Tp44); Tp44

Accession

CD28

Predicted N Terminal

Asn 19

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Molecular Mass

Recombinant Human CD28/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 375 amino acids and predictes a molecular mass of 42 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCD28/Fc monomer is approximately 55-65 kDa due to glycosylation.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 90 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Background

Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Enhances the production of IL4 and IL10 in T-cells in conjunction with TCR/CD3 ligation and CD40L costimulation (PubMed:8617933). Isoform 3 enhances CD40L-mediated activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (PubMed:15067037).

Tag

Species

Human

Source

HEK293

BACKGROUND

Background