Recombinant Human CD226 Protein(His tag) (HH0130CL)

Recombinant Human CD226 Protein (Q15762) (Met 1-Asn 247) with a polyhistidine tag at the C-terminus was expressed in HEK293.

Datasheet

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PRODUCT INFORMATION

Cat.No.

HH0130CL

Synonyms

CD226; DNAM 1; DNAM1; PTA1; TLiSA1; DNAM-1;

Accession

CD226

Predicted N Terminal

Glu 19

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by its binding ability in a functional ELISA . Immobilized human CD226 at 2 μg/ml (100 μl/well) can bind human CD112 with a linear ranger of 6.4-800 ng/ml.

Molecular Mass

Recombinant Human DNAM1 comprises 240 amino acids and has a predicted molecular mass of 27.6 kDa. As a result of glycosylation, rh DNAM1 migRates with an apparent molecular mass of 40-50 kDa in SDS-PAGE under reducing conditions.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 97 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Background

This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.

Tag

His

Species

Human

Source

HEK293

BACKGROUND

Background