Recombinant Human CD200 Protein(Fc-His tag) (HH0152CL)

Recombinant Human CD200 Protein (P41217) (Met 1-Gly 232) with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus was expressed in HEK293.

Datasheet

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PRODUCT INFORMATION

Cat.No.

HH0152CL

Synonyms

CD200; CD200 molecule; MRC; OX 2; MOX1; MOX2; OX-2;

Accession

CD200

Predicted N Terminal

Gln 31

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured by its binding ability in a functional ELISA. Immobilized human CD200R1 at 1 μg/ml (100 μl/well) can bind human CD200 Fc Chimera with a linear range of 0.12-16 ng/ml.

Molecular Mass

Recombinant Human CD200/Fc is a disulfide-linked homodimer. The reduced monomer consists of 450 amino acids and has a predicted molecular mass of 50.5 kDa. As a result of glycosylation, the apparent molecular mass of rh CD200/Fc monomer is approximately 65-70 kDa in SDS-PAGE under reducing conditions.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Tag

Fc-His

Species

Human

Source

HEK293