Recombinant Human CPA4 Protein(His tag) (HI0005CS)
Recombinant Human CPA4 Protein (NP_057436) (17-421aa) with C-terminus His tag was expressed in Insect cells.
| Size | Price | Qty |
|---|---|---|
| 50ug | $495.00 |
PRODUCT INFORMATION
Cat.No.
HI0005CS
Synonyms
CPA4; carboxypeptidase A4; carboxypeptidase A3; CPA3;
Accession
Form
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
Molecular Mass
46.6kDa (413aa)
40-57KDa (SDS-PAGE under reducing conditions.)
40-57KDa (SDS-PAGE under reducing conditions.)
AA Sequence
GQEKFFGDQV LRINVRNGDE ISKLSQLVNS NNLKLNFWKS PSSFNRPVDV LVPSVSLQAF KSFLRSQGLE YAVTIEDLQA LLDNEDDEMQHNEGQERSSN NFNYGAYHSL EAIYHEMDNI AADFPDLARR VKIGHSFENR PMYVLKFSTG KGVRRPAVWL NAGIHSREWI SQATAIWTARKIVSDYQRDP AITSILEKMD IFLLPVANPD GYVYTQTQNR LWRKTRSRNP GSSCIGADPN RNWNASFAGK GASDNPCSEV YHGPHANSEVEVKSVVDFIQ KHGNFKGFID LHSYSQLLMY PYGYSVKKAP DAEELDKVAR LAAKALASVS GTEYQVGPTC TTVYPASGSS IDWAYDNGIK
FAFTFELRDT GTYGFLLPAN QIIPTAEETW LGLKTIMEHV RDNLYLEHHH HHH
FAFTFELRDT GTYGFLLPAN QIIPTAEETW LGLKTIMEHV RDNLYLEHHH HHH
Endotoxin
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Purity
> 95% by SDS - PAGE
Storage
Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20Centigrade or -70Centigrade. Avoid repeated freezing and thawing cycles.
Concentration
1mg/ml (determined by Absorbance at 280nm)
Warning
For research use only!
Background
This gene is a member of the carboxypeptidase A/B subfamily, and it is located in a cluster with three other family members on chromosome 7. Carboxypeptidases are zinc-containing exopeptidases that catalyze the release of carboxy-terminal amino acids, and are synthesized as zymogens that are activated by proteolytic cleavage. This gene could be involved in the histone hyperacetylation pathway. It is imprinted and may be a strong candidate gene for prostate cancer aggressiveness.
References
Huang H., et al. (1999) Cancer Res. 59:2981-2988.
Pallares I., et al. (2005) Proc Natl Acad Sci USA. 102:3978-3983.
Pallares I., et al. (2005) Proc Natl Acad Sci USA. 102:3978-3983.
Tag
His tag
Species
Human
Source
Insect cells
BACKGROUND
Background
This gene is a member of the carboxypeptidase A/B subfamily, and it is located in a cluster with three other family members on chromosome 7. Carboxypeptidases are zinc-containing exopeptidases that catalyze the release of carboxy-terminal amino acids, and are synthesized as zymogens that are activated by proteolytic cleavage. This gene could be involved in the histone hyperacetylation pathway. It is imprinted and may be a strong candidate gene for prostate cancer aggressiveness.
References
Huang H., et al. (1999) Cancer Res. 59:2981-2988.
Pallares I., et al. (2005) Proc Natl Acad Sci USA. 102:3978-3983.
Pallares I., et al. (2005) Proc Natl Acad Sci USA. 102:3978-3983.
Reference Reading
1. Alonso del Rivero, Maday; Reytor, Mey L.; Trejo, Sebastian A.; et al. A Noncanonical Mechanism of Carboxypeptidase Inhibition Revealed by the Crystal Structure of the Tri-Kunitz SmCI in Complex with Human CPA4, STRUCTURE, 21-7, 1118-1126.
"The crystal structure of SmCI (Sabellastarte magnifica carboxypeptidase inhibitor) has been determined in complex with human carboxypeptidase A4 (hCPA4). SmCI is composed by three BPTI/Kunitz domains, each one displaying high structural homology and functionality with serine protease inhibitors."