Recombinant Cynomolgus PDGFB Protein(Fc tag) (CH0269PL)

Recombinant Cynomolgus PDGFB Protein (G7PFK7) (Ser82-Thr190) with the Fc region of mouse IgG1 at the N-terminus was expressed in HEK293.

Datasheet

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PRODUCT INFORMATION

Cat.No.

CH0269PL

Synonyms

PDGFB; Putative uncharacterized protein

Accession

PDGFB

Predicted N Terminal

Asp

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose and 5 % mannitol.

Bio-activity

Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 5-25 ng/ml. Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus PDGFRB-His at 10 μg/ml (100 μl/well) can bind Cynomolgus S4-mFc3-PDGFB, The EC50 of Cynomolgus S4-mFc3-PDGFB is 3.6-7.4 ng/ml.

Molecular Mass

Recombinant Cynomolgus PDGFB is a disulfide-linked homodimer. The reduced monomer comprises 345 amino acids and has a calculated molecular mass of 38.9 KDa. The apparent molecular mass of it is approximately 43 and 32 KDa in SDS-PAGE.

Endotoxin

< 1.0 EU per 1 microgram of protein (determined by LAL method).

Purity

> 95 % by SDS-PAGE.

Storage

In lyophilized state for 1 year (4°C); After reconstitution under sterile conditions for 3 months (-70°C). Avoid repeated freeze/thaw cycles.

Reconstitution

Reconstitute in sterile distilled water to a concentration of 0.1-1.0 mg/mL.

Warning

For research use only!

Background

Growth factor. Belongs to the PDGF/VEGF growth factor family.

Tag

Species

Cynomolgus

Source

HEK293

BACKGROUND

Background

Growth factor. Belongs to the PDGF/VEGF growth factor family.