Product description
Pyruvate plays an important role in various biochemical pathways, can be converted into carbohydrates during gluconeogenesis, or converted into fatty acids through acetyl CoA. Pyruvate reacts with 2,4-dinitrophenylhydrazine to form 2,4-dinitrophenylhydrazone, which turns brown-red in an alkaline solution; the content of pyruvate can be determined by reading the absorbance at 520nm.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, desktop centrifuge, pipette, mortar and pestle, ice, distilled water.