Pull-down Kit - RNA pull-down Kit (HZKIT007)

RNA pull-down is utilized to investigate the interactions between RNA molecules and proteins within cellular environments. This method involves the biotinylation of RNA, which is then mixed with cell lysate, leading to the formation of either RNA-RNA or RNA-protein complexes. These complexes are subsequently captured and isolated by incubating with streptavidin-coated magnetic beads. To ascertain the interactions between the target RNA and other RNA molecules, techniques such as quantitative fluorescence PCR (referred to as RNA pull-down-QPCR) or next-generation sequencing (known as RNA pull-down-seq) are employed. Furthermore, to identify protein interactions with the target RNA, methods like Western blot analysis (designated as pull-down-WB) and mass spectrometry (referred to as pull-down-MS) are applied.