Product description
Pectin is a major component of the primary cell wall and middle lamella, mainly in the form of protopectin, pectic acid methyl ester, and pectic acid, widely distributed in plant fruits, roots, and leaves. Pectin combines with cellulose and metal ions to form water-insoluble protopectin, which makes the fruit firm and crisp. This kit first extracts protopectin and uses the carbazole colorimetric method to determine the protopectin content. Protopectin is hydrolyzed into soluble pectin in dilute acid and further transformed into galacturonic acid, which undergoes a condensation reaction with carbazole in sulfuric acid solution to form a purple-red substance. The purple-red substance has a maximum absorption peak at 530nm upon spectral scanning, and the color depth is proportional to the pectin content, thus obtaining the protopectin content.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, water bath, pipettes, ethanol, concentrated sulfuric acid, mortar.