Product description
The BCA Protein Assay Kit provides a simple, rapid method for detecting protein concentration that is resistant to detergents (up to 5%). Since proteins can reduce Cu2+ to Cu+, BCA can bind with Cu+ to form a purple-blue complex, which has a maximum absorbance at 562nm. The color intensity is proportional to the protein content, allowing for the determination of protein concentration based on absorbance values.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvettes (optical path 1cm), desktop centrifuge, temperature-controlled water bath, pipettes.