Product description
Proline content in plants to a certain extent reflects plant stress resistance. Drought-resistant varieties tend to accumulate more proline. Therefore, determining proline content can serve as a physiological indicator for drought-resistant breeding. Additionally, due to its strong hydrophilicity, proline can stabilize protoplasm colloids and metabolic processes within tissues, thereby lowering the freezing point and preventing cell dehydration. Under low-temperature conditions, increased proline content in plant tissues can enhance cold resistance, making it a physiological indicator for cold-resistant breeding as well. When plantT are extracted with sulfosalicylic acid, proline becomes free in the solution, then reacts with acidic ninhydrin upon heating to form a red substance, which has a maximum absorption peak at 520nm. The content of free proline in plants can be determined through colorimetry.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, constant temperature water bath, desktop centrifuge, pipette, glacial acetic acid, mortar, ice