Product description
Phytase (EC.3.1.3.8) is a collective term for enzymes that catalyze the hydrolysis of phytic acid and its salts into inositol and phosphate (salts), belonging to the class of phosphomonoesterases. Phytase can convert phytic acid and its salts in food and feed into available phosphorus for organisms, reduce phosphorus content in feces, alleviate environmental pollution, and improve the absorption and utilization of nutrients, thus having extensive research and application value. Under certain temperature and pH conditions, phytase hydrolyzes the substrate sodium phytate to generate inorganic phosphorus and inositol derivatives. The inorganic phosphorus reacts with a specific color reagent to form a blue complex in an acidic environment. By measuring the rate of color development of this colored substance at 700nm, the activity of phytase can be calculated.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, balance, low-temperature centrifuge, temperature-controlled water bath, pipette.