Phenylalanine Ammonia-Lyase (PAL) Activity Assay Kit (OSRAP-K050M)

Cat.No.

OSRAP-K050M

Method

Microplate-based method

Product size

48T/96T

Product description

Phenylalanine ammonia-lyase (PAL, EC 4.3.1.24) is the enzyme that catalyzes the first step of the phenylpropanoid metabolic pathway and is a key and rate-limiting enzyme in this pathway. It is closely related to the synthesis of important secondary metabolites such as lignin, isoflavonoids, and flavonoid pigments. Studies have found that many plants activate their defense systems, especially the phenylpropanoid metabolism, when subjected to cold, injury, and UV radiation, leading to a rapid increase in PAL activity. Therefore, PAL activity can also serve as a physiological indicator of plant stress resistance. This kit measures PAL activity based on the cleavage of L-phenylalanine into trans-cinnamic acid and ammonia, with trans-cinnamic acid having maximum absorption at 290nm. PAL activity is calculated by measuring the rate of increase in absorbance.

Additional Materials and Equipments Required

Microplate reader, 96-well plate (UV plate), water bath, centrifuge, pipette, mortar, ice, and distilled water.