Sign In / Register          (0)

Phenylalanine Ammonia-Lyase (PAL) Activity Assay Kit (OSRAP-K050M)

PRODUCT INFORMATION

Cat.No.
OSRAP-K050M
Method
Microplate-based method
Product size
48T/96T
Product description
Phenylalanine ammonia-lyase (PAL, EC 4.3.1.24) is the enzyme that catalyzes the first step of the phenylpropanoid metabolic pathway and is a key and rate-limiting enzyme in this pathway. It is closely related to the synthesis of important secondary metabolites such as lignin, isoflavonoids, and flavonoid pigments. Studies have found that many plants activate their defense systems, especially the phenylpropanoid metabolism, when subjected to cold, injury, and UV radiation, leading to a rapid increase in PAL activity. Therefore, PAL activity can also serve as a physiological indicator of plant stress resistance. This kit measures PAL activity based on the cleavage of L-phenylalanine into trans-cinnamic acid and ammonia, with trans-cinnamic acid having maximum absorption at 290nm. PAL activity is calculated by measuring the rate of increase in absorbance.
Additional Materials and Equipments Required
Microplate reader, 96-well plate (UV plate), water bath, centrifuge, pipette, mortar, ice, and distilled water.
Online Inquiry

Fill out this form and one of our experts will respond to you within one business day.

We use cookies to understand how you use our site and to improve the overall user experience. This includes personalizing content and advertising. Read our Privacy Policy

Accept Cookies
x