Product description
Phenol oxidase (PO, EC 1.14.18.1), also known as tyrosinase, is a copper ion-binding oxidase. It plays a crucial role in insect immunity, cuticle tanning, melanization, and wound healing. PO can catalyze L-dopa to produce quinone, which has characteristic light absorption at 490nm. The PO enzyme activity can be determined by measuring the change rate at 490nm.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, desktop centrifuge, water bath, pipette, mortar, ice, and distilled water.