Product description
Pepsin is a digestive protease secreted by the chief cells of the gastric mucosa, breaking down proteins in food into small peptide fragments. It is generally used to differentiate neurological hypochlorhydria and is also reduced in secretion in chronic gastritis, chronic gastric dilation, and chronic duodenitis. Pepsin can catalyze the hydrolysis of hemoglobin to produce phenolic amino acids, which react with Folin-Ciocalteu reagent to show a blue color; the depth of the color is directly proportional to the activity of pepsin.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (optical path 1cm), desktop centrifuge, pipette, balance, mortar and pestle, ice, and distilled water.