Product description
Pectin esterase, belonging to the pectinase family, is also known as pectin methylesterase or pectin oxidase. It catalyzes the hydrolysis of methoxyl groups on pectin chains to produce small molecular substances of pectinic acid and methanol, thereby increasing the solubility of pectin in water. It is widely found in higher plants and microorganisms that can degrade cell walls, playing an endogenous regulatory role in the content of pectin on and between plant cell walls and cells. Pectin esterase (PE) catalyzes the hydrolysis of pectin molecules to release H+, causing the pH of the reaction system to drop. An alkali solution is used to maintain the pH of the system at 7.8 (phenolphthalein indicator remains pink), and the activity of pectin esterase is reflected by the consumption of NaOH.
Additional Materials and Equipments Required
Balance, mortar, centrifuge, water bath or temperature-controlled incubator.