Ornithine Transaminase (OAT) Assay Kit (PROAO-K012M)

Cat.No.

PROAO-K012M

Method

Microplate-based method

Product size

96T

Note

Provide microplate

Product description

Ornithine transaminase (OAT, EC 2.6.1.13) is a key enzyme in the synthesis of proline from ornithine and plays a crucial role in plants' adaptation to stress. Ornithine transaminase (OAT) catalyzes the deamination of ornithine in the presence of substrate ornithine and α-ketoglutarate, accompanied by the oxidation of NADH. By detecting the decrease in absorbance of NADH at 340nm, the enzyme activity of ornithine transaminase can be calculated.

Additional Materials and Equipments Required

Microplate reader, 96-well plate, water bath, centrifuge, pipette, mortar, ice, and distilled water.