Product description
Nitrate reductase (NR, EC 1.7.1.3) is widely present in plants and is an inducible enzyme. It is a key enzyme in the conversion of nitrate nitrogen to ammonium nitrogen in plants and is related to the effective absorption and utilization of nitrogen fertilizer by crops. It is one of the indicators of plant nutrition or field fertilization and can also be used as an indicator for variety selection. This kit uses an in vitro method to detect the reduction of nitrate to nitrite catalyzed by nitrate reductase, and further the nitrite reacts with sulfonamide and α-naphthylamine to form a purple-red azo compound. The color intensity is directly proportional to the activity of nitrate reductase, and the activity can be determined by measuring the absorbance at 530nm.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvettes (optical path 1cm), pipettes, water bath/temperature-controlled incubator, desktop centrifuge, mortar and pestle, ice, and distilled water.