Product description
Invertase, also known as sucrose invertase (E.C.3.2.1.26), catalyzes the breakdown of sucrose into fructose and glucose in sucrose metabolism and is one of the key enzymes in sucrose metabolism in higher plants. Based on the optimal pH values, sucrose invertases are divided into acid invertase (AI) and neutral invertase (NI), with many reports considering neutral invertase to be the same as alkaline invertase. NI has an optimal pH around 7.0 and is primarily found in the cytoplasm, where it is responsible for breaking down sucrose into fructose and glucose. NI catalyzes the decomposition of sucrose to produce reducing sugars, which then react with 3,5-dinitrosalicylic acid to form a brown-red amine compound with characteristic light absorption at 540nm. The light absorption value at 540nm is proportional to the amount of reducing sugars generated within a certain range. The activity of NI is calculated by the rate of increase in light absorption.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, low-temperature centrifuge, water bath, pipette, mortar and pestle, ice, and distilled water.