Product description
Sorbitol dehydrogenase (SDH) catalyzes the dehydrogenation of sorbitol to fructose and is one of the key enzymes regulating the content of sorbitol in organisms. Depending on the coenzyme it depends on, it is divided into NADP+-dependent sorbitol dehydrogenase (NADP+-SDH, EC 1.1.1.B60) and NAD+-dependent sorbitol dehydrogenase (NAD+-SDH). NADP+-SDH catalyzes the conversion of sorbitol to glucose, while NAD+-SDH catalyzes the conversion of sorbitol to fructose. NADP+-SDH catalyzes the dehydrogenation of sorbitol to produce glucose and reduces NADP+ to NADPH. The increase in absorbance at 340nm can be used to calculate the activity of NADP+-SDH.
Additional Materials and Equipments Required
UV spectrophotometer, 1mL quartz cuvette (light path 1cm), desktop centrifuge, water bath, pipette, mortar and pestle, ice, and distilled water.