Product description
Glutamate dehydrogenase is widely distributed in living organisms and plays an important role in the assimilation of ammonia and the transformation into organic nitrogen compounds. Its coenzymes are NADH or NADPH, both of which exist in animals and plants, with NADH-glutamate dehydrogenase (EC 1.4.1.2) being predominant. This kit provides a rapid and sensitive detection method; NADH-glutamate dehydrogenase in the sample specifically acts on the substrate glutamate to produce NADH and reacts with a color developer to form a yellow substance, which has a maximum absorption peak at 450nm, allowing for the determination of NADH-GDH enzyme activity.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, desktop centrifuge, water bath, pipettes, mortar and pestle, ice, and distilled water.