Product description
NADK (EC 2.7.1.23) is widely present in animals, plants, microorganisms, and cultured cells, and is the only enzyme found in organisms that can catalyze the phosphorylation of NAD+ to NADP+. Therefore, NAD kinase plays an important role in the synthesis of NADP(H) and the regulation of the balance between NAD(H) and NADP(H). NADK catalyzes the phosphorylation of NAD+ to NADP+; then, under the action of 6-phosphogluconate dehydrogenase and 6-phosphogluconate, NADP+ is reduced to NADPH. The enzyme activity is determined by measuring the increase rate of NADPH at 340 nm.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, low-temperature desktop centrifuge, pipette, mortar and pestle, and distilled water.