Product description
Complex I (EC 1.6.5.3), also known as NADH-CoQ reductase or NADH dehydrogenase, is widely present in the mitochondria of animals, plants, microorganisms, and cultured cells, and is the largest protein complex in the mitochondrial inner membrane. The enzyme catalyzes the transfer of a pair of electrons from NADH to CoQ, and can also reduce O2 to O2.-, which is the main site for the production of O2.- in the respiratory electron transport chain. Measuring the enzyme activity can reflect the state of the respiratory electron transport chain (ETC) and the generation of reactive oxygen species (ROS). Complex I can catalyze the dehydrogenation of NADH to NAD+, and the enzyme activity can be determined by measuring the oxidation rate of NADH at 340nm.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, pipette, low-temperature desktop centrifuge, mortar and pestle, ice, and distilled water.