Product description
Mitochondrial-3-phosphoglycerate dehydrogenase (mt-GPD) is located in the mitochondria and plays a crucial role in the 3-phosphoglycerate pathway, catalyzing the substrate 3-phosphoglycerate to form phosphodihydroxyacetone, while the generated electrons and hydrogen enter the respiratory chain to participate in oxidative phosphorylation. In the presence of the electron carrier (PMS), it reduces thiazolyl blue (MTT) to form a blue product. By detecting the increase rate of the blue product at 550nm, the activity of mt-GPD can be determined.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, pipette, desktop centrifuge, water bath, mortar, ice, and distilled water.