Product description
Mitochondrial-3-phosphoglycerate dehydrogenase (mt-GPD) is located in the mitochondria and plays an important role in the 3-phosphoglycerate pathway, catalyzing the substrate 3-phosphoglycerate to form dihydroxyacetone phosphate, while the generated electrons and hydrogen enter the respiratory chain to participate in oxidative phosphorylation. In the presence of the electron carrier (PMS), it reduces thiazolyl blue (MTT) to form a blue product. By measuring the increase in the blue product at 550nm, the activity of mt-GPD can be determined.
Additional Materials and Equipments Required
Visible spectrophotometer, quartz cuvette (optical path 1cm), pipette, desktop centrifuge, water bath, mortar, ice, and distilled water.