Product description
In dairy production, the term "urea nitrogen" commonly refers to milk urea nitrogen (MUN), which mainly originates from the degradation of proteins in the rumen. Measuring MUN is of profound significance for maintaining the nitrogen balance in dairy cows, maximizing production potential, and efficiently utilizing feed proteins to reduce costs. This kit utilizes the hydrolysis of urea by urease to produce ammonium ions and carbon dioxide. The ammonium ions react with a phenolic color developer in an alkaline medium to form a blue substance, the amount of which is proportional to the urea content. The urea nitrogen (MUN) content in milk can be determined by measuring the colored substance at 625nm.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, balance, water bath, pipettes, centrifuge, distilled water.