Mg2+-ATPase is related to the maintenance of intracellular Mg2+ concentration in cells and can catalyze the hydrolysis of ATP to produce ADP and inorganic phosphate while transporting Mg2+. The enzyme activity is determined by measuring the amount of inorganic phosphate.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (optical path 1cm), water bath, desktop centrifuge, pipette, mortar and pestle, ice, and distilled water.