Product description
Limonin, a representative of triterpenoid compounds, is the main cause of "post-bitterness" in citrus fruits during processing. Enzymatic conversion to reduce the content of limonin is a suitable method for the juice industry. The activity of limonin convertase is proportional to the consumed content of limonin. Limonin forms a stable red complex with Ehrlich's reagent, and the change in absorbance OD value can be measured to determine the activity of limonin convertase.
Additional Materials and Equipments Required
Microplate reader, 96-well plate (UV plate), pipette, balance, low-temperature centrifuge