Product description
Leucine dehydrogenase (LeuDH, EC 1.4.1.9) is an NAD+-dependent oxidoreductase that can catalyze the reversible reaction of L-leucine and branched-chain L-amino acids to produce the corresponding α-keto acids and their analogs. This kit utilizes leucine dehydrogenase (LeuDH) to catalyze the reaction of 2-ketobutyric acid and NADH to produce aminobutyric acid and NAD+. By measuring the decrease rate of NADH at 340nm, the activity of leucine dehydrogenase (LeuDH) can be calculated.
Additional Materials and Equipments Required
UV spectrophotometer, 1mL quartz cuvettes (optical path 1cm), desktop centrifuge, pipettes, mortar, ice, and distilled water.