Product description
Leucine aminopeptidase (LAP, EC 3.4.11.1) is a protease that hydrolyzes peptide chains with leucine at the N-terminus and is widely present in tissues such as the liver, kidneys, and pancreas, especially abundant in the liver. LAP decomposes L-leucyl p-nitroanilide to produce p-nitroaniline, which has a maximum absorption peak at 405nm. By measuring the rate of increase in absorbance, the activity of LAP can be calculated.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvettes (optical path 1cm), desktop centrifuge, pipettes, balance, mortar, ice, and distilled water.