Product description
The L-galactose pathway is recognized as the primary pathway for the synthesis of ascorbate (AsA) in plants. L-Galactono-1,4-lactone dehydrogenase (L-galactono-1,4-lactone dehydrogenase, GaLDH, EC1.3.2.3) is located in the inner mitochondrial membrane and directly oxidizes L-galactono-1,4-lactone (L-galactono-1,4-lactone, Gal) to produce AsA, which is the key enzyme in the last step of the plant AsA biosynthetic pathway. Gal LDH catalyzes the reduction of L-galactono-1,4-lactone by cytochrome C (Cyt c), and the reduced Cyt c has an absorption peak at 550nm; the activity of Gal LDH can be calculated by measuring the increase rate of reduced Cyt c.
Additional Materials and Equipments Required
Plate reader, 96-well plate, desktop centrifuge, pipette, mortar and pestle, ice, and distilled water.