Product description
The L-galactose pathway is recognized as the primary pathway for the synthesis of ascorbate (AsA) in plants. L-Galactono-1,4-lactone dehydrogenase (L-galactono-1,4-lactone dehydrogenase, GaLDH, EC1.3.2.3) is located in the inner mitochondrial membrane and directly oxidizes L-galactono-1,4-lactone (L-galactono-1,4-lactone, Gal) to produce AsA, which is the key enzyme in the last step of the plant AsA biosynthetic pathway. Gal LDH catalyzes the reduction of L-galactono-1,4-lactone by cytochrome C (Cyt c), and the reduced Cyt c has an absorption peak at 550nm; the activity of Gal LDH can be calculated by measuring the increase rate of reduced Cyt c.
Additional Materials and Equipments Required
Visible spectrophotometer, 1mL glass cuvette (light path 1cm), desktop centrifuge, pipette, mortar and pestle.