Product description
Pectin is a major component of the primary cell wall and middle lamella, primarily present in the form of protopectin, pectic acid methyl ester, and pectic acid, and is widely distributed in plant fruits, roots, and leaves. Pectin is cross-linked with other substances through Ca2+, Mg2+, hydrogen bonds, glycosidic bonds, and ester bonds. Specific extraction methods can be used to extract ionically bound pectin (ISP) and covalently bound pectin (CSP). This kit specifically extracts ionically bound pectin (ISP) with an acid solution containing a chelating agent and uses the sulfuric acid-carbazole colorimetric method to determine the pectin content. Pectin is hydrolyzed to galacturonic acid, which undergoes a condensation reaction with carbazole in sulfuric acid solution to form a purple-red substance. The substance has a maximum absorption peak at 530nm upon spectral scanning, and the color depth is proportional to the pectin content, thus obtaining the content of ionically bound pectin.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, water bath, pipettes, ethanol, concentrated sulfuric acid, mortar.