Product description
The glyoxalase system is the primary pathway for the removal of methylglyoxal (MG), and glyoxalase II (Gly II, EC 3.1.2.6) is an enzyme in the glyoxalase system, widely expressed in mammals, plants, and bacteria. Glyoxalase II catalyzes the hydrolysis of S-D-lactoylglutathione (SLG) into reduced glutathione (GSH) and D-lactic acid. Reduced glutathione (GSH) reacts with DTNB to form a yellow complex, which has a characteristic absorption peak at 412nm. By measuring the rate of increase at 412nm, the activity of glyoxalase II (Gly II) can be determined.
Additional Materials and Equipments Required
Microplate reader (wavelength set to 412nm), 96-well plate, pipette, low-temperature centrifuge, mortar