Glyoxalase I (Gly I) Activity Assay Kit (OSRAG-K006M)

Cat.No.

OSRAG-K006M

Method

Microplate-based method

Product size

96T

Product description

The glyoxalase system is the primary pathway for the removal of methylglyoxal (MG), and glyoxalase I (Gly I, EC 4.4.1.5) is a cytosolic enzyme that makes up the glyoxalase system. Glyoxalase I catalyzes the formation of S-D-lactoylglutathione (SLG) from methylglyoxal (MG) and reduced glutathione, which has a characteristic absorption peak at 240nm. By measuring the increase in absorbance at 240nm, the activity of glyoxalase I (Gly I) can be calculated.

Additional Materials and Equipments Required

Microplate reader (wavelength set to 240nm), 96-well UV plate, pipette, mortar, distilled water