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Glyoxalase I (Gly I) Activity Assay Kit (OSRAG-K005S)

PRODUCT INFORMATION

Cat.No.
OSRAG-K005S
Method
Spectrophotometry
Product size
48T
Product description
The glyoxalase system is the primary pathway for the removal of methylglyoxal (MG), and glyoxalase I (Gly I, EC 4.4.1.5) is a cytosolic enzyme that makes up the glyoxalase system. Glyoxalase I catalyzes the formation of S-D-lactoylglutathione (SLG) from methylglyoxal (MG) and reduced glutathione, which has a characteristic absorption peak at 240nm. By measuring the increase in absorbance at 240nm, the activity of glyoxalase I (Gly I) can be calculated.
Additional Materials and Equipments Required
UV spectrophotometer (wavelength set to 240nm), 1mL quartz cuvette (light path 1cm), pipette, mortar, distilled water
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