Product description
Glycogen synthase (GCS, EC 2.4.1.11) adds UDP-G sugar residues to glucose residues, extending the sugar chain with α-1,4-glycosidic bonds. GCS is the rate-limiting enzyme in glycogen synthesis and plays an important role in sugar metabolism and the maintenance of blood glucose homeostasis. GCS catalyzes the reaction between UDPG and glucose residues to form glycogen and UDP. Under the successive action of pyruvate kinase and lactate dehydrogenase, NADH is oxidized to NAD+. By detecting the decrease in NADH at 340nm, the GCS enzyme activity can be calculated.
Additional Materials and Equipments Required
Microplate reader, 96-well plate, desktop centrifuge, pipette, mortar and pestle, ice, and distilled water.